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Identification and Molecular Characterization of Nocardia sp. as a New Causal Agent of Tobacco False Broomrape |
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| Authors: | Yunior Morán Osmani Chacón Maria del Carmen Córdoba‐Sellés Rosario Domínguez‐Larrinaga Lidcay Herrera Orlando Borrás‐Hidalgo |
| Affiliation: | 1. Instituto de Investigaciones del Tabaco, , San Antonio de los Ba?os, Artemisa, Cuba;2. Universidad Politécnica de Valencia (UPV), , Valencia, 46022 Spain;3. Universidad Central “Marta Abreu” de las Villas, , Santa Clara, Villa Clara, Cuba;4. Centro de Ingeniería Genética y Biotecnología (CIGB), , Playa, La Habana, Cuba |
| Abstract: | Tobacco false broomrape disease is a serious problem in tropical countries. To identify its cause, experiments were conducted in tobacco fields. Six actinomycete strains were isolated from white succulent outgrowths of tobacco roots and their pathogenicity was confirmed by biological testing. Based on phenotypic and 16S rRNA gene sequence BLAST analysis, the strains were identified as members of the genus Nocardia. This association was also confirmed by secA1 gene phylogenetic analysis. This is the first report of Nocardia sp. as the cause of tobacco false broomrape. |
| Keywords: | Nicotiana tabacum Nocardia false broomrape secA1 16S rRNA Cuba |