Expression of the CMV‐CP Gene in Synechocystis 6803 Affects Cyanobacterial Photosynthesis |
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Authors: | Chun‐Mei Wang Ding‐Ji Shi Shui‐Fang Zhu Yin Yie Po Tien Ning‐Sheng Wei |
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Affiliation: | 1. Beijing University of Chinese Medicine, , Beijing, 100102 China;2. College of Plant Protection, Northwest Agriculture and Forestry University, , Yangling, Shanxi, 712100 China;3. Institute of Botany, Chinese Academy of Science, , Beijing, 100093 China;4. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, , Beijing, 100029 China;5. Institute of Microbiology, Chinese Academy of Science, , Beijing, 100101 China |
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Abstract: | Previous work has shown that the presence of excess coat protein (CP) of cucumber mosaic virus (CMV) in the chloroplasts was related with mosaic symptoms. However, whether these mosaic symptoms are directly induced by the interaction between CP and chloroplasts is unknown. To directly demonstrate the interaction between CP and the chloroplast, Synechocystis sp. PCC 6803 was used as the chloroplast model. The cDNA encoding the CMV‐CP was cloned in a cyanobacterial shuttle vector (pKT‐CP) and transferred to Synechocystis sp. PCC 6803. The CP was expressed in the cyanobacterium with the psbA promoter. The expression of CMV‐CP hindered the growth of transgenic cyanobacterium cells and decreased its photosynthetic rate and the PS II activity. The transgenic cells showed increased fluorescence (F) from the phycobilisome terminal emitters and increased fluorescence (F) from PS II. The absorption spectra at room temperature showed the Chl and the phycocyanin absorption peak of the mutant strain significantly decreased. These results showed that CP may directly affect the cyanobacterium cells and decreased its photosynthesis, especially the PS II activity. These data might provide new evidence for mosaic symptoms being directly induced by the interaction between CP and chloroplasts. |
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Keywords: |
Cucumber mosaic virus
coat protein photosynthesis transgenic cyanobacterium Synechocystis sp. PCC 6803 |
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