Copper and Manganese Increase Resistance of Clusterbean to Root Rot Caused by Rhizoctonia |
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Authors: | Neha Wadhwa Udai Narayan Joshi Sudarshan Kumar Gandhi |
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Affiliation: | 1. Department of Biochemistry, CCS Haryana Agricultural University, , Hisar, 125004 Haryana, India;2. Department of Pathology, CCS Haryana Agricultural University, , Hisar, 125004 Haryana, India |
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Abstract: | Clusterbean seedlings growing in soil inoculated with Rhizoctonia were treated with either 5 or 10 ppm Cu2+ and Mn2+. Soil was inoculated by pretreatment with 250 mg (wet weight) of Rhizoctonia inoculum. A similar set of plants was maintained in uninoculated soil. Root rot incidence of plants treated with Cu2+ 5 ppm, Cu2+ 10 ppm, Mn2+ 5 ppm and Mn2+ 10 ppm was 26.6, 30.5, 11.8 and 29.2% less than the inoculated control, respectively. Inoculation with Rhizoctonia reduced chlorophyll, non‐structural carbohydrate and in vitro dry matter digestibility (IVDMD) content compared with uninoculated ones. Oxidative enzymes activities (polyphenol oxidase, peroxidase, phenylalanine ammonia lyase and tyrosine ammonia lyase), crude protein, phenolic content, structural components (acid detergent fibre, cellulose and lignin), silica, macronutrients and micronutrients increased in inoculated seedlings and this increase was further heightened by the Cu2+ 10 ppm treatment compared with the Cu2+ 5 ppm, Mn2+ 5 ppm and Mn2+ 10 ppm treatments in response to fungal invasion. It was concluded that the Cu2+ 10 ppm treatment may be an effective soil nutrient to provide enhanced resistance of clusterbean plants to root rot (fungal) diseases. |
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Keywords: | Clusterbean root rot
Rhizoctonia
copper manganese micronutrients biochemical parameters |
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