Growth yield increase and ATP formation linked to succinate decarboxylation in Veillonella parvula |
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Authors: | Peter H Janssen |
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Institution: | 1. Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton, New Zealand
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Abstract: | Veillonella parvula strain 259 (=DSM 2007) was able to grow on a mineral salts medium supplemented with (per litre) 1 g yeast extract, 1 g Tween-80,
and 3 mg putrescine. 2 HCl, with 6 mM thioglycolate as reductant and lactate as growth substrate. Succinate did not serve
as a growth substrate, but when added in conjunction with lactate, it was decarboxylated to propionate and resulted in a measurable
increase in growth yield, corresponding to the formation of 2.4 g cell dry mass per mol succinate. A growth yield increase
linked to succinate metabolism occurred only while lactate was also being metabolised. Experiments with cell suspensions showed
that succinate decarboxylating activity was constitutive. Addition of succinate produced clear increases in cellular ATP levels
in ATP-depleted washed cells. |
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Keywords: | Succinate ATP formation Growth Veillonella Transmembrane gradient Decarboxylation Cometabolism |
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