Identification of the DNAs of the ectomycorrhizal fungusTricholoma bakamatsutake using specific oligonucleotide probes and PCR primers |
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Authors: | Yoshie Terashima Takao Nakai |
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Institution: | (1) Chiba Prefectural Forest Experiment Station, Haniya 1887-1, Sanbu-machi, 289-12 Sanbu-gun, Chiba, Japan;(2) The Institute of Physical and Chemical Research (RIKEN), Kouyadai 3-1-1, 305 Tsukuba-shi, Ibaraki, Japan |
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Abstract: | Partial nucleotides of the 18S rDNAs ofTricholoma bakamatsutake were sequenced and compared with those of six ectomycorrhizal fungi and a tree. Two probes, Probes 1 and 2, and a pair of
primers were designed based on the variable positions in this region. The DNAs ofT. bakamatsutake were isolated from the colonized mycelia in the soil, field-collected fruit-bodies and artifically cultured mycelia. Hybridization
with Probe 1 and PCR-amplification with the primers differentiated these DNAs of this fungus from those of eight ectomycorrhizal
fungi and two tree species. |
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Keywords: | ectomycorrhizal fungi hybridization PCR ribosomal DNA Tricholoma bakamatsutake |
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