A simple method for the screening of lactic acid bacteria for the production of exopolysaccharides in liquid media |
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Authors: | Ricciardi Annamaria Parente Eugenio Clementi Francesca |
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Affiliation: | (1) Dipartimento di Biologia, Difesa e Biotecnologie Agro-forestali, Universit della Basilicata, Via Anzio 10, 85100 Potenza, Italy |
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Abstract: | A rapid and convenient screening metnod for the production of exopolysaccharides (EPS) from lactic acid bacteria based on the measurement of ef_ux time in Microhaematocrit capillaries was developed. The traditional pick test was more sensitive in detecting evidence of polysaccharide production on solid media but did not re_ect the potential for EPS production in liquid media. Measurement of ef_ux time in Microhaematocrit capillaries provided quantitative results which were correlated with viscosity measurements and EPS concentration and were amenable to statistical analysis. |
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