Impact of MK-886 on H2O2 Generation by Human Neutrophils and Cell Degranulation |
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Authors: | I V Gorudko A V Timoshenko |
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Institution: | (1) Belarusian State University, pr. F. Skaryny 4, Minsk, 220050, Belarus;(2) Institute of Photobiology, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220072, Belarus |
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Abstract: | Metabolic inhibitors can clearly affect different aspects of the functional activity of cells. This property was studied in the present work with respect to MK-886, a well-known inhibitor of the 5´-lipoxygenase-activating protein. It was found that this inhibitor in a micromolar concentration range (2-20 µM) induced in a dose-dependent manner H2O2 generation by human neutrophils and the release of lysozyme from the cells. The MK-886-induced activation of neutrophils was accompanied by a significant decrease in N-(1-pyrene)maleimide-accessible SH-groups in the cells. According to its activity, MK-886 can be considered an agonist that causes up-regulation of inherent neutrophil functions. In summary, the results indicate that during the application of MK-886 as a 5´-lipoxygenase inhibitor in neutrophils, the impact of the compound on the functional activity of the cells should be taken into consideration. |
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Keywords: | MK-886 neutrophil lipoxygenase hydrogen peroxide degranulation lysozyme |
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