家蝇幼虫抗菌肽的抗菌谱及其与抗生素的协同作用研究

研究3种家蝇幼虫抗菌肽的抗菌谱以及每种抗菌肽的最小抑菌浓度(MIC),初步探讨3种抗菌肽分别与青霉素、链霉素相结合后对抗菌活性的影响,并采用分级抑制浓度指数(Fractionalinhibitoryconcentrationindex,FIC)来定量检测抗菌肽与抗生素之间的抗菌作用关系。结果表明3种抗菌肽的抗菌谱不同,不同的抗菌肽对不同病原菌的抗菌活性不同。3种抗菌肽与链霉素、青霉素之间的抗菌协同关系因细菌种类不同。抗菌肽与抗生素之间并不是都存在协同关系,有些不相关,甚至表现为对抗关系,表明抗菌肽、抗生素与细菌三者的相互作用关系非常复杂。

Antibacterial spectrum of antibacterial peptides from Musca dommestica larvae and synergic interaction between the peptides and antibiotics

In vitro antibacterial activities of three antibacterial peptides, alone and in combination with the antibiotics were measured by MIC and antibacterial spectrum. Synergy was observed when the peptides were combined with penicillin, streptomycin by FIC. The results show that antibacterial peptides have stronger inhibition of pathogenic bacterium, and antibacterial spectrum are very different. The interaction in antibacterial peptides, antibiotics and bacterium is extraordinary complex, except synergic action, there are antagonism and indifference.