| 糖化酶一个主要组分的纯化和有关光谱的测定 |
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| 引用本文： | 张海龙, 关国华, 孙世魁, 李壮, 黄程芳,. 糖化酶一个主要组分的纯化和有关光谱的测定[J]. 微生物学通报, 1996, 23(4): 206-209 |
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| 作者姓名： | 张海龙 关国华 孙世魁 李壮 黄程芳 |
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| 作者单位： | 吉林大学分子生物学系; |
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| 摘 要： | 采用糖化酶粗酶制剂，经抽提、硫铵分级和ＤＥＡＥ－纤维素层析等程序纯化了糖化酶一个主要组分，并经电泳鉴定被纯化至均一状态，分子量为８９０００。于不同条件下，测定了该组分的圆二色性谱和荧光发射谱，取得了关于该组分构象的一些重要结果。
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| 关 键 词： | 糖化酶 纯化 圆二色性谱 荧光发射谱 |
PURIFICATION AND RELATIVE SPECTRA OF GLUCOAMYLASE WITH MOLECULAR WEIGHT OF 89KD |
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| Zhang Hailong, Guan Guohua, Shun Shikui Li Zhuang, Huang Chengfang. PURIFICATION AND RELATIVE SPECTRA OF GLUCOAMYLASE WITH MOLECULAR WEIGHT OF 89KD[J]. Microbiology China, 1996, 23(4): 206-209 |
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| Authors: | Zhang Hailong Guan Guohua Shun Shikui Li Zhuang Huang Chengfang |
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| Abstract: | in this study, a primary component of Glucoamylase was purified by extraction, ammonium sulfate fractionation, DEAE-cellulose 52 chromatography, purity examined by PAGE was shown by the presence of a single band. In the presence of different concentrations of Ca2 ,iodoacetic acid or urea, circular dichroism spectra and nuorescence spectra of this component were measured. In the experiment conditions, each ligands could change the conformation of this component. These results could offer some important information about the conformation of this component. |
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| Keywords: | Glucoamylase Purification Circular dichroism spectra Fluorescence spectra |
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