Characterization of an HPV-negative cell line (FR-car) derived from a cervical squamous intraepithelial lesion |
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| Authors: | Carolien van Haaften-Day Barbara Rose Carol Thompson Robyn Lukeis Peter Russell |
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| Institution: | (1) Department of Gynaecological Oncology, Royal Hospital for Women, 2021 Sydney, NSW, Australia;(2) Department of Infectious Diseases, University of Sydney, 2006, NSW, Australia;(3) Kanematsu Laboratories, Royal Prince Alfred Hospital, 2050 Sydney, NSW, Australia;(4) Department of Anatomical Pathology, Royal Prince Alfred Hospital, 2006 Sydney, NSW, Australia;(5) Research Unit, Royal Hospital for Women, 2031 Randwick, Sydney, NSW, Australia |
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| Abstract: | Summary A new cell line, FR-car, has been established from a biopsy of a low-grade human cervical squamous intraepithelial lesion
(SIL). We confirmed the epithelial origin of the cells by keratin staining using polykeratin, AE1/AE3 and CAM 5.2 antibodies.
Sixty percent to 80% of the cultured cells stained positive for proliferative cell nuclear antigen (PCNA) and Ki-67. There
was no overexpression of p53. Karyotyping revealed that the cell line was hypodiploid with clonal abnormalities on chromosome
6 and 16. Sections of a biopsy adjacent to the lesion from which the culture was initiated tested positive for human papillomavirus
(HPV) 18 DNA by the polymerase chain reaction, but cultured cells tested at several passages were HPV-negative by either type-specific
or consensus PCRs. This HPV-negative SIL line may be useful in studies into the cell biology of dysplastic epithelium. |
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| Keywords: | cervix squamous intraepithelial neoplasia immunohistochemistry polymerase chain reaction human papillomavirus |
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