Induced diploid gynogenesis and polyploidy in the ornamental (koi) carp Cyprinus carpio L. |
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Authors: | N B Cherfas Y Peretz N Ben-Dom B Gomelsky G Hulata |
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Institution: | (1) Agricultural Research Organization, Fish and Aquaculture Research Station, Dor, Mobile Post Hof Hacarmel, 30820, Israel |
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Abstract: | The results of a series of experiments conducted in our laboratory on the ornamental common carp (koi), aimed at optimizing heat-shock chromosome-set manipulation procedures, are described. The timing of heat-shock initiation was expressed in the relative unit of embryological age ( 0) in order to standardize this parameter, the absolute time for heat-shock initiation being calculated from duration of one 0 at two different pre-treatment water temperatures. Heat shocks were applied within the periods of 0.05–0.60 0 and 1.20–2.20 0 which, respectively, cover the successive phases of the 2nd meiotic division and the 1st cleavage. The highest production of diploid gynogenetic offspring was observed when heat shocks were initiated at 0.15–0.25 0 and at 1.5 0, after insemination, corresponding to anaphase of meiosis-II, and metaphase of the 1st cleavage, respectively. Similar results were obtained irrespective of the different pre-treatment water temperatures, thus confirming the possibility of standardizing heat-shock timing by 0. |
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Keywords: | Common carp Chromosome-set manipulations Heat-shock timing Induced gynogenesis |
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