CD8+ cells protect mice against reinfection with the intestinal parasite Eimeria falciformis |
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| Authors: | Thomas Pogonka Katja Schelzke Jörg Stange Konstantin Papadakis Svenja Steinfelder Oliver Liesenfeld Richard Lucius |
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| Institution: | 1. Department of Molecular Parasitology, Humboldt University Berlin, Germany;2. Department of Microbiology and Hygiene, Immunology of Infection Group, Charité Medical School, Berlin, Germany;3. German Rheumatology Research Center, Berlin, Germany;1. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, PIN-243122, India;2. Department of Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, AL97TA, UK;1. College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Korea;2. Department of Pediatrics, University of Maryland, School of Medicine, Baltimore, MD 21201, USA;3. Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA;1. Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India;2. Section of Immunology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India;1. Department of Hematology, Institute of Molecular Hematology, The Second Xiang-ya Hospital, Central South University, Changsha, Hunan, PR China;2. Division of Cancer Immunotherapy, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Kashiwanoha, Kashiwa, Chiba, Japan;3. Department of Cardiovascular Surgery, The Second Xiang-ya Hospital, Central South University, Changsha, Hunan, PR China;1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi''an 710069, China;2. Institute of Integrated Medical Information, Xi''an 710016, China;3. Department of Neurosurgery, Shanghai Tenth People''s Hospital, Shanghai 200072, China |
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| Abstract: | We investigated cellular immune responses of mice infected with the apicomplexan parasite Eimeria falciformis in order to characterise protective immune mechanisms and effector functions. Adoptive transfer experiments with mesenterial lymph node cells (MLNC) from immune donor mice were performed, and the oocyst output monitored after challenge infection. Phenotypical analysis by fluorescence cytometry and T cell proliferation assay showed that already from day four post infection E. falciformis-specific lymphocytes were present in the MLN. The frequency of parasite-specific, IFN-γ producing CD4+ and CD8+ cells increased in this period by 9.8% and 16.4%, respectively. Infection experiments with IFN-γ deficient mice revealed that IFN-γ is involved in resistance to primary and secondary infection. Transfer of total MLNC from immune donors reduced the oocyst output by 65–74%, as compared to the oocyst output of animals transferred with cells from naïve donors. Transfer of CD8+ cells inhibited parasite development resulting in a reduction of oocyst numbers by 42–64%, whereas CD4+ cells showed no influence on resistance to reinfection. |
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