Clinical isolates of Enterococcus faecalis aggregate human platelets |
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| Authors: | Magnus Rasmussen Daniel Johansson Sara K Söbirk Matthias Mörgelin Oonagh Shannon |
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| Institution: | 1. Department of Clinical Sciences, Division of Infection Medicine, Lund University, Tornavägen 10, BMC B14, 221 84 Lund, Sweden;2. Department of Clinical Microbiology, University and Regional Laboratories, 221 85 Lund, Sweden;1. Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan;2. Department of Internal Medicine, The University of Tokyo Hospital, Tokyo, Japan;1. Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland;2. Christine Kühne–Center for Allergy Research and Education, Davos, Switzerland;3. Department of Internal Medicine, Jagiellonian University, Krakow, Poland;1. School of Biosciences and Biotechnology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia;2. Institute of Ecology and Environmental Studies, Obafemi Awlowo University, Ile-Ife, Nigeria;3. School of Environmental & Natural Resource Sciences, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia;4. School of Chemical Sciences and Food Technology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia;1. Humboldt-Universität zu Berlin, Breeding Biology and Molecular Genetics, Invalidenstr. 42, D-10115 Berlin, Germany;2. Freie Universität Berlin, Department of Veterinary Medicine, Institute of Veterinary Anatomy, Koserstraße 20, 14195 Berlin, Germany;3. Federal Institute for Risk Assessment, Department of Biological Safety, Unit Molecular Diagnostics and Genetics, Diedersdorfer Weg 1, 12277 Berlin, Germany;1. Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia;2. Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia;1. Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico;2. Hospital Civil de Guadalajara, Fray Antonio Alcalde, and Instituto de Patología Infecciosa y Experimental, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico;3. Departamento de Patología Clínica, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Mexico;4. Laboratorio de Microbiología, Hospital General de Durango, Secretaria de Salud, Durango, Mexico;5. Serviciode Gastroenterología, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Mexico |
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| Abstract: | Many endocarditis pathogens activate human platelets and this has been proposed to contribute to virulence. Here we report for the first time that many clinical isolates of Enterococcus faecalis, a common pathogen in infective endocarditis, aggregate human platelets. 84 isolates from human blood and urine were screened for their ability to aggregate platelets from four different donors. Platelet aggregation occurred for between 11 and 65% of isolates depending on the donor. In one donor, a significantly larger proportion of isolates from blood than from urine caused platelet aggregation. Median time to aggregation was 11 min and had a tendency to be shorter for blood isolates as compared to urine isolates. Immunoglobulin G (IgG) was shown to be essential in mediating activation and aggregation. Platelet aggregation could be abolished by an IgG-specific proteinase (IdeS), by an antibody blocking FcRγIIa on platelets, or by preabsorption of plasma with an E. faecalis isolate. Fibrinogen binding to bacteria or platelets does not contribute to platelet activation or aggregation under our experimental conditions. These results indicate that platelet activation and aggregation by E. faecalis is dependent on both host and bacterial factors and that it may be involved in the pathogenesis of invasive disease with this organism. |
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