Molecular identification of two Trichogramma species (Hymenoptera: Trichogrammatidae) in Korea |
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Authors: | Gilsang Jeong Hwangyong Kim Youngcheol Choi Wontae Kim Kwanho Park Sungwoo Bae Jonggill Kim Jiyoung Choi |
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Affiliation: | 1. Department of Agricultural Biology, National Academy of Agricultural Science (NAAS), RDA, Suwon, 441-853, Republic of Korea;2. International Technology Cooperation Center, Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea |
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Abstract: | Trichogramma wasps parasitize eggs of various insect species. Several Trichogramma species have been commercialized to manage Lepidopteran agricultural pests. Correct species identification is essential for successful biological control. However, the microscopic size and morphological similarity of Trichogramma species makes correct identification very difficult. Internal transcribed spacer (ITS)-2 gene sequences have been used to identify Trichogramma species. Using this molecular marker, Trichogramma species that are commercially available in Korea were identified as Trichogramma ostriniae and Trichogramma brassicae. The latter has not previously been reported in Korea. Additionally, Cadra cautella and Sitotroga cereallela eggs (host eggs of the commercialized Trichogramma species in Korea) were infected with Wolbachia. However, Trichogramma were not infected with the bacterium. This indicates that horizontal transfer of Wolbachia does not occur from host to wasp. |
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