Nitrite uptake by Chlamydomonas reinhardtii cells immobilized in calcium alginate |
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Authors: | Carlos Vílchez José M. Vega |
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Affiliation: | (1) Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Apartado 553, E-41080 Sevilla, Spain |
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Abstract: | When an initial cell loading of about 30–40 µg chlorophyll (Chl)·g–1 gel and alginate suspension of 3% (w/v) were used for immobilization of Chlamydomonas reinhardtii, the resulting cell beads showed optimum nitrite uptake rate, at 30° C and pH 7.5, of 14 µmol NOinf2sup–·mg–1 Chl·h–1, the photosynthetic and respiratory activities being about 120 µmol O2 produced·mg–1 Chl·h–1, and 40 µmol O2 consumed ·mg–1 Chl·h–1, respectively. The nitrite uptake activity required CO2 in the culture and persisted after 8 days of cells immobilization, or in the presence of 0.2 mm ammonium in the medium. Our data indicate that alginate-entrapped C, reinhardtii cells may provide a stable and functional system for removing nitrogenous contaminants from waste-waters.Correspondence to: C. Vílchez |
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