Calmodulin binding proteins in rat liver mitochondria |
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Authors: | P Gazzotti M Gloor E Carafoli |
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Institution: | Dept. of Biochemistry, Swiss Federal Institute of Technology, (ETH) Universitaestrasse. 16, 8092 Zurich, Switzerland |
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Abstract: | Calmodulin binding proteins have been found in submitochondrial fractions obtained from highly purified rat liver mitochondria. The matrix fraction contains two major calmodulin binding proteins: one, having Mr of 145,000, apparently is carbamoyl-phosphate synthetase. Another has a Mr of 58,000 and has not been associated with enzyme activities. A major calmodulin binding protein of unknown function and having Mr of 32,000 has been found in the Triton X-100 solubilizate of the inner membrane. Minor amounts of two calmodulin binding proteins having Mr of about 37,000 and 56,000 have been found in the outer membrane. |
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Keywords: | BSA bovine serum albumin SDS sodium dodecylsulfate Hepes N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid PMSF phenylmethylsulfonyl fluoride EDTA Ethylendiaminetetraacetic acid EGTA Ethyleneglycol-bis-(amino-ethylether) N N -tetraacetic acid |
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