Manganese as a mutagenic agent during in vitro DNA synthesis. |
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Authors: | D K Dube L A Loeb |
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Institution: | The Institute for Cancer Research The Fox Chase Cancer Center 7701 Burholme Avenue Philadelphia, Pa. 19111, USA |
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Abstract: | The effect of Mn2+, a known mutagen, on the fidelity of DNA synthesis by avian myeloblastosis DNA polymerase has been determined. Substitution of Mn2+ for Mg2+ leads to an enhanced incorporation of noncomplementary deoxynucleotides as well as complementary ribonucleotides with either poly (A) or poly (C) as templates. Since this polymerase lacks any detectable deoxyribonuclease activity, the mutagenic effect of Mn2+ in promoting errors in base-pairing does not result from any diminished proof-reading function. |
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