Induction of anti-actin drug resistance in Tetrahymena |
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Authors: | Zackroff Robert V Hufnagel Linda A |
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Institution: | Massachusetts College of Pharmacy and Health Sciences, School of Arts and Sciences, 179 Longwood Avenue, Boston, MA 02115 USA. rzackroff@mcp.edu |
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Abstract: | Both cytochalasin D and latrunculin B reversibly inhibited Tetrahymena phagocytosis at concentrations similar to those effective in mammalian systems, even though ciliate actins are known to be highly divergent from mammalian actins. Overnight exposure to relatively low (0.25 microM) concentrations of latrunculin B induced resistance in Tetrahymena to the inhibitory effects of that drug, as well as cross-resistance to cytochalasin D. However, much higher (> 30 microM) concentrations of cytochalasin D were required for induction of cross-resistance to latrunculin B. Anti-actin drug resistance in Tetrahymena may involve a general multidrug resistance mechanism and/or specific feedback regulation of F-actin assembly and stability. |
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Keywords: | Actin ciliates cytochalasin cytoskeleton endocytosis food vacuoles latrunculin microfilaments multidrug resistance phagocytosis |
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