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| 香蕉离体茎尖超低温保存研究 | |
| 引用本文： | 李俊慧,何平,陈晓玲,卢新雄,辛霞,张志娥,辛萍萍. 香蕉离体茎尖超低温保存研究[J]. 植物遗传资源学报, 2010, 11(1): 34-39 |
| 作者姓名： | 李俊慧 何平 陈晓玲 卢新雄 辛霞 张志娥 辛萍萍 |
| 作者单位： | 1. 西南大学生命科学学院,重庆,400715;中国农业科学院作物科学研究所,北京,100081 2. 西南大学生命科学学院,重庆,400715 3. 中国农业科学院作物科学研究所,北京,100081 |
| 基金项目： | "十一五"国家科技支撑计划，中央级公益性科研院所基本科研业务费专项资助项目，农业部农作物种质资源保护利用专项资助项目 |
| 摘 要： | 以香蕉(Musaspp.)试管苗为试材,对其离体茎尖小滴玻璃化法超低温保存的影响因素进行了研究。小滴玻璃化法和玻璃化法超低温保存后再生率的差异表明,香蕉更适合用小滴玻璃化法进行超低温保存。香蕉小滴玻璃化法超低温保存的方案如下:试管苗在60g/L蔗糖的MS培养基上培养1~2个月,剥离带有1~2片叶原基的茎尖,室温下装载30m in(可延长至4h),0℃下PVS2处理40~50m in。6个基因型的14个品种的再生率平均为46.9%。通过SSR分子标记检测,再生植株的遗传稳定性没有发生改变。该结果为香蕉种质资源的长期保存提供了理论依据和技术支撑。 |
| 关 键 词： | 香蕉 离体茎尖 小滴玻璃化法 超低温保存 遗传稳定性 |
Cryopreservation of in Vitro Shoot-Tips of Banana(Musa spp.) |
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| LI Jun-hui,,HE Ping,CHEN Xiao-ling,LU Xin-xiong,XIN Xia,ZHANG Zhi-e,XIN Ping-ping. Cryopreservation of in Vitro Shoot-Tips of Banana(Musa spp.)[J]. Journal of Plant Genetic Resources, 2010, 11(1): 34-39 | |
| Authors: | LI Jun-hui HE Ping CHEN Xiao-ling LU Xin-xiong XIN Xia ZHANG Zhi-e XIN Ping-ping |
| Affiliation: | LI Jun-hui1,2,HE Ping1,CHEN Xiao-ling2,LU Xin-xiong2,XIN Xia2,ZHANG Zhi-e2,XIN Ping-ping2 (1 School of Life Science,Southwest University,Chongqing 400715,2 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081) |
| Abstract: | In vitro shoot-tips of banana(Musa spp.)were successfully cryopreserved by droplet vitrification method.The factors affecting the post-thaw regeneration of shoot tips were analyzed.The results showed that the droplet vitrification method was more suitable for cryopreservation of banana in vitro shoot-tips than vitrification method.The optimal procedures were as follows:Shoot-tips with 1 or 2 leaf primordia excised from rooted in vitro plantlets which were cultured on MS medium supplemented with 60g/L sucros... |
| Keywords: | Banana In vitro shoot-tip Droplet vitrification method Cryopreservation Genetic stability |
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