Increased protein content in transgenic Arabidopsis thaliana over-expressing nitrate reductase activity |
| |
| Affiliation: | 1. Environmental Microbiology Unit, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of The Negev, Sede Boqer Campus 84993, Israel;2. Albert Katz Center for Desert Agrobiology, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of The Negev, Sede Boqer Campus 84993, Israel;1. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China;2. University of Chinese Academy of Sciences, Beijing, 100049, PR China;3. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, PR China;1. Institute for Molecular Biotechnology, Worringerweg 1, RWTH Aachen University, 52074 Aachen, Germany;2. Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstraße 6, 52074 Aachen, Germany;1. University of Agriculture in Kraków, Faculty of Agriculture and Economics, Department of Plant Physiology, Podłużna 3, 30-239 Kraków, Poland;2. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland;1. Membrane Protein Biology Group, ICGEB, Aruna Asaf Ali Marg, New Delhi, Delhi 110067, India;2. Department of Biotechnology, Jamia Hamdard University, New Delhi, Delhi, 110062, India;3. Crop Improvement Group, ICGEB, Aruna Asaf Ali Marg, New Delhi, Delhi, 110067, India;4. Department of Medical Biochemistry, HIMSR, Jamia Hamdard University, New Delhi, Delhi, 110062, India;1. CAS Key Laboratory of Computational Biology, Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;3. Department of Biochemistry, Department of Plant Biology, and Center of Biophysics and Quantitative Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA |
| |
| Abstract: | Arabidopsis thaliana was engineered to over-express nitrate reductase (NR) by virtue of the light inducible chimeric gene Lhcb1*3::Nia1*2. The transgenic lines obtained displayed NR activity 2–4 times the level in the wild type, depending on the line. While not displaying advantage with respect to fresh or dry weight, 7-day-old transgenic seedlings did show up to 200% higher protein content than the wild type when grown on solid medium. Up to 30% increase in protein content was also obtained when grown in peat moss for at least 3 weeks. The increase in protein content was evident in several protein bands but was most apparent in that of the large subunit of RuBisCo. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
