EFFECTS OF DIETARY Spirulina maxima ON ENDOTHELIUM DEPENDENT VASOMOTOR RESPONSES OF RAT AORTIC RINGS |
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| Institution: | 1. Department of Biochemistry, School of Medicine, UNAM. P.O. Box 70-159, Mexico D.F. 04510, MEXICO;1. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, GD 510640, China;2. Institute of Ocean and Earth Sciences, University of Malaya, 50603, Kuala Lumpur, Malaysia;3. Institute for Advanced Studies, University of Malaya, 50603, Kuala Lumpur, Malaysia;4. Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama, 790 8577, Ehime Prefecture, Japan;5. Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei, 11529, Taiwan;6. Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia;7. China-ASEAN College of Marine Sciences, Xiamen University Malaysia, 43900, Sepang, Selangor, Malaysia;8. Laboratory of Microbial Ecology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;1. Department of Aquatic Ecotoxicology, Faculty of Biological Sciences, Goethe University Frankfurt am Main, Max-von-Laue-Str. 13, 60438 Frankfurt am Main, Germany;2. Department of Biology, Norwegian University of Science and Technology, 5 Høgskoleringen, 7491 Trondheim, Norway |
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| Abstract: | The aim of this study was to evaluate the effects of Spirulina maxima on vasomotor responses of aorta rings from male Wistar rats fed on a purified diet. For this purpose, the animals (weighing 200–240 g) were allocated randomly in two groups. One receiving purified control diet (A) and the other receiving purified diet containing 5% Spirulina (B). Purified diets were according to American Institute of Nutrition guidelines and adjusted to Spirulina protein content. All animals were fed (20 g/day/rat) during two weeks, receiving water ad libitum and 12 h. lightdark cycles. Spirulina maxima effects were evaluated by concentration-response (CR) curves of aorta rings with or without endothelium to phenylephrine (PE), both in presence and absence of indomethacin (Indom) or indomethacin plus L-NAME (Indom. + L-NAME), and to carbachol (CCh). Aorta rings with endothelium from group B showed, relative to corresponding rings from group A: 1) a significant decrease in the maximal tension developed in response to PE. 2) this decrease was reverted by Indom. 3) Indom. + L-NAME induced an additional increase in the contractile responses to PE. 4) a significant shift to the left of the CR curve to CCh. No significant differences were observed in the tension developed in response to PE in rings without endothelium from either group. These results suggest that Spirulina maxima may decrease vascular tone by increasing the synthesis and release of both a vasodilating cyclooxygenase-dependent product of arachidonic acid and nitric oxide, as well as by decreasing the synthesis and release of a vasoconstricting eicosanoid from the endothelial cells. © 1997 Elsevier Science Inc. |
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