Ultrasound permeabilizes CHO cells for the endonucleases AluI and benzon nuclease |
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| Institution: | 1. Department of Animal and Range Sciences, New Mexico State University, Las Cruces, NM 88003, USA;2. Extension Animal Sciences and Natural Resources, New Mexico State University, Las Cruces, NM 88003, USA |
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| Abstract: | Ultrasound permeabilizes Chinese hamster ovary (CHO) cells for the endonucleases AluI and benzon nuclease which leads to the induction of chromosomal aberrations by these enzymes. A few aberrant cells were observed when trypsinized cells or adherent cells were exposed to the enzymes in the absence of ultrasound. Our data show that sonication can be used to introduce endonucleases into CHO cells. We further demonstrate that few cells can internalize endonucleases without previous permeabilization. |
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