Extra hepatic formation of bilirubin glucuronides in the rat

After total hepatectomy in the rat, the presence of conjugated bilirubin in the plasma has been demonstrated using an extraction technique. This is particularly striking after loading the animals with unconjugated bilirubin. The identification of bilirubin glucuronide has been achieved using thin-layer chromatography of the azopigments formed (1) with ethyl anthranilate and (2) with p-iodoaniline.