Synthesis of the vasoactive intestinal peptide (VIP) I. The C-terminal cyanogen bromide fragment |
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| Authors: | Miklos Bodanszky Yakir S Klausner Viktor Mutt |
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| Institution: | 1. Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106 USA;2. Department of Biochemistry, Medicinska Nobelinstitutet, Karolinska Institutet, Stockholm, Sweden |
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| Abstract: | The C-terminal cyanogen bromide fragment of VIP, Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2 (all l), was synthesized to provide evidence for the correctness of the sequence proposed by Mutt and Said (1). The synthesis of this hendecapeptide (VIP18–28) was carried out by coupling Z-Ala-Val-(Z)Lys to (Z)Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2. After removal of the protecting groups and purification, the synthetic material was indistinguishable from the natural fragment on paper chromatograms and electropherograms. Their identity was further confirmed by comparison of the products formed on enzymatic hydrolysis. |
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