Characterization of the mannosyl and fucosyl transferases in the ovine anterior pituitary glands. |
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| Authors: | C Tertrin-Clary M P De La Llosa-Hermier P De La Llosa |
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| Institution: | Laboratoire des Hormones Polypeptidiques, C.N.R.S., 91190 Gif-sur-Yvette, France |
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| Abstract: | Ovine anterior pituitary glands contain mannosyl- and fucosyl-transferases localized in the microsomes and able to incorporate mannose or fucose as such from GDP-mannose or GDP-fucose into endogenous glycoproteins. The requirements and conditions necessary for maximum activity were investigated. The value of the Km is very similar for the two enzyme systems, 3 X 10(-7) M in the case of mannosyl-transferases and 5 X 10(-7) M in the case of fucosyl-transferases. |
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| Keywords: | GDP-mannose guanosine diphosphomannose GDP-fucose guanosine diphosphofucose |
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