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Direct sequencing of PCR products using the Maxam-Gilbert method |
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| Authors: | S Stamm F M Longo |
| Institution: | Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029. |
| Abstract: | Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method. |
| Keywords: | |