Isolation and developmental characterization of temperature-sensitive carrot cell variants |
| |
Authors: | G. Giuliano F. Lo Schiavo M. Terzi |
| |
Affiliation: | (1) Istituto di Mutagenesi e Differenziamento, C.N.R., Via Svezia 10, I-56100 Pisa, Italy |
| |
Abstract: | Summary Carrot cell lines multiply indefinitely in the presence of the auxin 2,4-D. If auxin is removed, the cells regenerate plantlets in a process that closely resembles embryogenesis in vivo. We isolated a temperature-sensitive variant, ts 2, which is unable to regenerate at 31 °C (non-permissive temperature), but does form embryos and plants at 24 °C (permissive temperature). The temperature treatment had no effect on fully differentiated ts 2 plantlets. In other variants (ts 5 and ts 11) cell proliferation was inhibited at the restrictive temperature. These lines were leaky with respect to the inhibition of embryogenesis at 31 °C.Abbreviations EMS ethylmethanesulfonate - EU embryogenic unit (see Materials and methods) - ts temperature-sensitive - 2,4-D 2,4-dichlorophenoxyacetic acid |
| |
Keywords: | Differentiation Carrot Somatic embryogenesis Temperature sensitivity |
本文献已被 SpringerLink 等数据库收录! |
|