Role of the Aquaporin PIP1 Subfamily in the Chilling Tolerance of Rice |
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Authors: | Matsumoto, Tadashi Lian, Hong-Li Su, Wei-Ai Tanaka, Daisuke Liu, Cheng wei Iwasaki, Ikuko Kitagawa, Yoshichika |
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Affiliation: | 1Graduate School of Bioresource Sciences, Akita Prefectural University, Shimoshinjo, Akita, 010-0195 Japan 2Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China |
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Abstract: | Although an association between chilling tolerance and aquaporinshas been reported, the exact mechanisms involved in this relationshipremain unclear. We compared the expression profiles of aquaporingenes between a chilling-tolerant and a low temperature-sensitiverice variety using real-time PCR and identified seven genesthat closely correlated with chilling tolerance. Chemical treatmentexperiments, by which rice plants were induced to lose theirchilling tolerance, implicated the PIP1 (plasma membrane intrinsicprotein 1) subfamily member genes in chilling tolerance. Ofthese members, changes in expression of the OsPIP1;3 gene suggestedthis to be the most closely related to chilling tolerance. AlthoughOsPIP1;3 showed a much lower water permeability than membersof the OsPIP2 family, OsPIP1;3 enhanced the water permeabilityof OsPIP2;2 and OsPIP2;4 when co-expressed with either of theseproteins in oocytes. Transgenic rice plants (OE1) overexpressingOsPIP1;3 showed an enhanced level of chilling tolerance andthe ability to maintain high OsPIP1;3 expression levels underlow temperature treatment, similar to that of chilling-tolerantrice plants. We assume that OsPIP1;3, constitutively overexpressedin the leaf and root of transgenic OE1 plants, interacts withmembers of the OsPIP2 subfamily, thereby improving the plantswater balance under low temperatures and resulting in the observedchilling tolerance of the plants. |
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Keywords: | Aquaporin - Caffeine - Chilling tolerance - OsPIP1 - Rice - Transgenic |
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