Construction in vitro of "phage-plasmid" chimerae: a new tool to analyse the mechanism of plasmid maintenance. |
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Authors: | M Couturier J Janssens F Bex A Desmyter and I Bonnevalle |
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Institution: | (1) Laboratory of Genetics, Department of Molecular Biology, Université libre de Bruxelles, rue des Chevaux, 67, B-1640 Rhode St Genèse, Belgium |
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Abstract: | Summary In this paper, we report the construction in vitro of chimerae between lambdoid replacement vectors (Murray et al., 1977) and the miniF Apr plasmid: pSC138 (Timmis et al., 1975). F recombinants were shown to be chimerae between the and the F replicons. By genetical tests, we have demonstrated that both and F replication mechanisms are functional: the F recombinant behaves as a non defective plaque forming phage on sensitive bacteria and establishes itself as a stable plasmid on recA F- homoimmune bacteria. In the extra-chromosomal state, the F recombinant apparently retains the controlled autonomous replication and the FI incompatibility characteristics of the F plasmid. The potential experimental uses of these phages are discussed. |
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