Preparation of polyvinyl alcohol-polyacrylamide composite polymer membrane by gamma-irradiation for entrapment of urease |
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| Authors: | Jha Sandeep Kumar D'Souza Stanislaus F |
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| Affiliation: | Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India. |
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| Abstract: | Composite polymer membrane of polyvinyl alcohol (PVA) and acrylamide was prepared on cheesecloth support by gamma-irradiation induced free radical polymerization. The enzyme urease was entrapped in the membrane during polymerization and was cross-linked within the matrix using glutaraldehyde. The membranes could be reused a number of times without significant loss of urease activity. |
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| Keywords: | Polyvinyl alcohol Polyacrylamide γ-irradiation Urease Polymer membrane Entrapment |
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