Oocyte-directed depletion of connexin43 using the Cre-LoxP system leads to subfertility in female mice |
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Authors: | Gershon Eran Plaks Vicki Aharon Idan Galiani Dalia Reizel Yitzhak Sela-Abramovich Sagit Granot Irit Winterhager Elke Dekel Nava |
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Institution: | a Leibniz Institute für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466, Gatersleben, Germany b Leibniz Institute für Biochemie der Pflanzen, 06120 Halle/Saale, Germany c BASF Plant Science GmbH, 67117 Limburgerhof, Germany |
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Abstract: | A T-DNA insertion mutant of FUSCA3 (fus3-T) in Arabidopsis thaliana exhibits several of the expected deleterious effects on seed development, but not the formation of brown seeds, a colouration which results from the accumulation of large amounts of anthocyanin. A detailed phenotypic comparison between fus3-T and a known splice point mutant (fus3-3) revealed that the seeds from both mutants do not enter dormancy and can be rescued at an immature stage. Without rescue, mature fus3-3 seeds are non-viable, whereas those of fus3-T suffer only a slight loss in their germinability. A series of comparisons between the two mutants uncovered differences with respect to conditional lethality, in histological and sub-cellular features, and in the relative amounts of various storage compounds and metabolites present, leading to a further dissection of developmental processes in seeds and a partial reinterpretation of the complex seed phenotype. FUS3 function is now known to be restricted to the acquisition of embryo-dependent seed dormancy, the determination of cotyledonary cell identity, and the synthesis and accumulation of storage compounds. Based on DNA binding studies, a model is presented which can explain the differences between the mutant alleles. The fus3-T lesion is responsible for loss of function only, while the fus3-3 mutation induces various pleiotropic effects conditioned by a truncation gene product causing severe mis-differentiation. |
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