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Levels of membrane fluidity in the spinal cord and the brain in an animal model of amyotrophic lateral sclerosis |
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| Authors: | Francisco Javier Miana-Mena Eduardo Piedrafita Cristina González-Mingot Pilar Larrodé María Jesús Muñoz Enrique Martínez-Ballarín Russel J. Reiter Rosario Osta Joaquín J. García |
| Affiliation: | 1. Department of Pharmacology and Physiology, Universidad de Zaragoza, Zaragoza, Spain 2. Neurology Service, Universitary Hospital Lozano Blesa of Zaragoza, Zaragoza, Spain 4. Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, USA 3. LAGENBIO, Universidad de Zaragoza, Zaragoza, Spain 5. Departamento de Farmacología y Fisiología, Facultad de Medicina de Zaragoza, C/Domingo Miral s/n, 50009, Zaragoza, Spain |
| Abstract: | A mutant form of the copper/zinc superoxide dismutase (SOD1) protein is found in some patients with amyotrophic lateral sclerosis (ALS). Alteration of the activity of this antioxidant enzyme leads to an oxidative stress imbalance, which damages the structure of lipids and proteins in the CNS. Using fluorescence spectroscopy, we monitored membrane fluidity in the spinal cord and the brain in a widely used animal model of ALS, the SODG93A mouse, which develops symptoms similar to ALS with an accelerated course. Our results show that the membrane fluidity of the spinal cord in this animal model significantly decreased in symptomatic animals compared with age-matched littermate controls. To the best of our knowledge, this is the first report showing that membrane fluidity is affected in the spinal cord of a SODG93A animal model of ALS. Changes in membrane fluidity likely contribute substantially to alterations in cell membrane functions in the nervous tissue from SODG93A mice. |
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