Sensitivity of Streptococcus thermophilus to Chemical Permeabilization |
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Authors: | George A Somkuti Mary E Dominiecki Dennis H Steinberg |
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Institution: | (1) U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Philadelphia, PA 19118, USA , US |
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Abstract: | Streptococcus thermophilus cultures were treated with conjugated and unconjugated bile salts and tested for β-galactosidase activity. Na-deoxycholate
and chenodeoxycholate were more efficient permeabilizing agents than cholate, and all three bile salts were superior to their
corresponding glyco- and tauro-conjugates. Treatment with sodium dodecyl sulfate resulted in the highest measurable β-galactosidase
levels in permeabilized cells, whereas response to Triton X-100 was variable and strain dependent. Na-deoxycholate, chenodeoxycholate,
and sodium dodecyl sulfate caused cell injury and arrested culture growth for 4 h or longer. The nongrowing permeabilized
biomass of S. thermophilus was used to hydrolyze lactose in aqueous solutions and milk. |
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