Hollow Microneedle Arrays for Intradermal Drug Delivery and DNA Electroporation |
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Authors: | Liévin Daugimont Nolwenn Baron Gaëlle Vandermeulen Natasa Pavselj Damijan Miklavcic Marie-Caroline Jullien Gonzalo Cabodevila Lluis M Mir Véronique Préat |
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Institution: | 1. Louvain Drug Research Institute, Unité de pharmacie galénique, Université Catholique de Louvain, Avenue E. Mounier 73/20, 1200, Brussels, Belgium 2. Département MN2S, Institut FEMTO-ST, UMR CNRS 6174, ENSMM, Besan?on, France 3. Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia 4. CNRS UMR 8029, SATIE, ENS Cachan Bretagne, Bruz, France 5. CNRS UMR 8203, Institut Gustave-Roussy, Villejuif and Université Paris-Sud, Orsay, France
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Abstract: | The association of microneedles with electric pulses causing electroporation could result in an efficient and less painful
delivery of drugs and DNA into the skin. Hollow conductive microneedles were used for (1) needle-free intradermal injection
and (2) electric pulse application in order to achieve electric field in the superficial layers of the skin sufficient for
electroporation. Microneedle array was used in combination with a vibratory inserter to disrupt the stratum corneum, thus
piercing the skin. Effective injection of proteins into the skin was achieved, resulting in an immune response directed to
the model antigen ovalbumin. However, when used both as microneedles to inject and as electrodes to apply the electric pulses,
the setup showed several limitations for DNA electrotransfer. This could be due to the distribution of the electric field
in the skin as shown by numerical calculations and/or the low dose of DNA injected. Further investigation of these parameters
is needed in order to optimize minimally invasive DNA electrotransfer in the skin. |
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