Effects of oligosaccharins on callus growth and saponin content of Panax notoginseng

This work provides some evidences for the saponin production of Panax notoginseng callus by using biologically active, wall-related oligosaccharins. In an appropriate concentration, three kinds of oligosaccharins stimulated saponin formation or callus growth. The concentration of DO, GO and CO for saponin production of Panax notoqinseng callus culture were 15ppm, 15ppm aud 20ppm respectively by comparing saponin yield. It was very obvious for DO to increase saponin content when the concentration was 10ppm, and for GO to stimulate callus growth when the concentration was 20ppm. It would be a good way to produce saponin by using oligosaccharins in large scale culture in the future.