A proteomic analysis of powdery mildew (Blumeria graminis f.sp. hordei) conidiospores |
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Authors: | SANDRA NOIR,THOMAS COLBY,ANNE HARZEN,JÜ RGEN SCHMIDT, RALPH PANSTRUGA |
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Affiliation: | Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany |
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Abstract: | Conidiospores are the asexual propagation units of many plant-pathogenic fungi. In this article, we report an annotated proteome map of ungerminated conidiospores of the ascomycete barley powdery mildew pathogen, Blumeria graminis f.sp. hordei . Using a combination of two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, we have identified the proteins in 180 spots, which probably represent at least 123 distinct fungal gene products. Most of the identified proteins have a predicted function in carbohydrate, lipid or protein metabolism, indicating that the spore is equipped for the catabolism of storage compounds as well as for protein biosynthesis and folding on germination. |
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