Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA polymerases during PCR |
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| Authors: | Kuwahara Masayasu Takahata Yumi Shoji Atsushi Ozaki Akiko N Ozaki Hiroaki Sawai Hiroaki |
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| Affiliation: | Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515, Japan. |
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| Abstract: | In order to enhance a collection of modified deoxynucleoside triphosphates useful for in vitro selection or SELEX (systematic evolution of ligands by exponential enrichment) techniques, we designed and synthesized modified analogues of 2'-deoxyuridine triphosphate and 2'-deoxycytidine triphosphate bearing a flexible and hydrophilic 7-amino-2,5-dioxaheptyl linker at a C5 position. Both analogues were found to be substrates for thermostable DNA polymerases which belong to an evolutional family B during PCR. |
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