Regulation of LAT52 promoter activity during pollen tube growth through the pistil of Nicotiana alata. |
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Authors: | P D Gerola C A Mol E Newbigin W M Lush |
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Institution: | (1) Department of Structural and Functional Biology, University of Insubria, via Henry Dunant 3, Varese, Italy E-mail: paolo.gerola&unimi.it; Fax: +39-332-421331, IT;(2) Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia, AU;(3) Laboratorium voor Plantenveredeling, Landbouwuniversiteit, Wageningen, The Netherlands, NL |
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Abstract: | The pollen-specific promoter of the LAT52 gene is known to direct expression of marker proteins during the last stages of
pollen maturation and in very early pollen tube growth.We have examined the expression of LAT52-GUS during later stages of
pollen tube growth in style and ovary of the relatively long-styled species Nicotiana alata. GUS activity was detected histochemically and found to be present in germinating pollen grains of N. alata and in tubes growing through the upper part of the style. No GUS activity was detected in 99% of the pollen tubes growing
through the lower part of the style, but activity was present in tubes within the ovary. This finding indicates that the LAT52
promoter is regulated in growing pollen tubes, and is most active during the earliest and latest stages of pollen tube growth.
GUS activity was also detected in some ovules, where it presumably marked the release of pollen tube cytoplasm into the ovule.
The distribution of ovules with GUS activity within the ovary is not consistent with high-precision pollen tube guidance to
the ovule.
Received: 16 August 1999 / Revision accepted: 20 December 1999 |
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