Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings |
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Authors: | G J Kumari A M Reddy S T Naik S G Kumar J Prasanthi G Sriranganayakulu P C Reddy Chinta Sudhakar |
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Institution: | (1) Department of Botany and Biotechnology, Sri Krishnadevaraya University, Anatapur, 515003, India;(2) Institute of Plant Genetics and Crop Plant Research, Gatersleben, D-06466, Germany;(3) College of Pharmacy, Chungbuk National University, Cheongju, 361-763, South Korea |
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Abstract: | Protein pattern, ammonia content, glutamine synthetase activity, lipid peroxidation, superoxide dismutase, catalase, peroxidase
and peroxidase isoforms were studied in the leaves and roots of 7-d-old peanut (Arachis hypogaea L. cv. JL-24) seedlings treated by 25, 100 and 250 μM jasmonic acid (JA). SDS-PAGE protein profile of leaves and roots after
JA application showed a significant increase in 18, 21, 30, 45, 47 and 97.4 kDa proteins and significant decrease in 22 and
36 kDa proteins. Pathogenesis related PR-18 was specific in leaves at 250 μM JA and PR-21 have cross reacted differently with
21 and 30 kDa proteins in leaves and roots treated by all JA concentrations. Further, the immunoblot analysis with glutamine
synthetase, GS-45 antibodies revealed a specific cross reaction with 45 and 47 kDa proteins of both control and JA treated
leaves, however, higher at 100 and 250 μM JA treated leaves than control ones. Further, the malondialdehyde (MDA) content
significantly increased in leaves and roots treated with JA, indicated membrane damage with JA treatments that led to the
generation of peroxidation products. The peroxidase isozymic pattern showed two specific isoforms. Besides, the activities
of SOD and catalase were significantly elevated in JA treated leaves. |
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Keywords: | Arachis hypogaea catalase lipid peroxidation PR-proteins SDS-PAGE |
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