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THE BIOSTIMULATORY EFFECT OF RED LASER IRRADIATION ON PIG BLOOD PLATELET FUNCTION
Authors:MONIKA OLBAN  BARBARA WACHOWICZ  MARIA KOTER  MARIA BRYSZEWSKA
Institution:1. Department of Biophysics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland;2. Department of General Biochemistry, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland;1. Centre for Heart Rhythm Disorders, University of Adelaide and Royal Adelaide Hospital, Adelaide, Australia;2. Department of Public Health, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon;3. Health Information Management and Risk Assessment Unit, World Health Emergencies Programme, World Health Organization Region for Africa, Kinshasa, Democratic Republic of the Congo;4. Diabetes Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, United States;5. Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon;6. Department of General Medicine, Lafe-Baleng Divisional Hospital, Western Regional Delegation, Ministry of Public Health, Bafoussam, Cameroon;7. National Perinatal Epidemiology Unit, Nuffield Department of Population Health, University of Oxford, Oxford, United Kingdom;8. Department of Research, Cameroon Society of Epidemiology, Yaoundé, Cameroon;9. School of Public Health, Faculty of Medicine, University of Paris-Sud, Le Kremlin-Bicêtre, France;10. School of Health and Related Research, The University of Sheffield, Sheffield, United Kingdom;11. Department of Epidemiology and Public Health, Centre Pasteur of Cameroon, Yaoundé, Cameroon;1. College of Food Science and Technology & MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China;2. College of Food and Biotechnology, Wuhan Institute of Design and Science, Wuhan 430205, China;3. School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China;4. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;1. Food Processing Research Center, Korean Food Research Institute, Seongnam 13539 Republic of Korea;2. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05030 Republic of Korea;3. Meat Science and Muscle Biology Lab, Purdue University, West Lafayette, IN 47907, USA;1. Department of Physical Therapy for Women’s Health, Faculty of Physical Therapy, Cairo University, Giza, Egypt;2. Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University, Giza, Egypt;1. National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 200240 Shanghai, PR China;2. Jiangxi Ganfeng Lithium Co., Ltd, 338004 Xinyu, Jiangxi, PR China
Abstract:The molecular mechanisms of laser-induced changes in the cell structure and function are not well known. The authors examined the effects of low-power laser irradiation on unnucleated pig blood platelets. The obtained results showed that laser irradiation (1–5J) caused in blood platelets lipid peroxidation (measured as thiobarbituric acid reactive substances) and super-oxide anion generation, concomitant with the release of adenine nucleotides and proteins from platelets. The maximum platelet response to laser irradiation was observed when doses of 1.8–2J were used. Our results indicate that red laser irradiation induces: (1) platelet secretory process and the release of substances stored in the specific granules (adenine nucleotides, proteins); and (2) lipid peroxidation partly due to stimulation of endogenous arachidonate and production of its metabolites reacting with thiobarbituric acid.
Keywords:pig blood platelet  laser  biostimulating effect
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