Structural and Immunochemical Studies of Two Cross-Reactive Proteus mirabilis O-Antigens,O6 and O23, Containing β1→3-Linked 2-Acetamido-2-Deoxy-d-Glucopyranose Residues |
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Authors: | Maciej Cedzyski Anna St
wierzko Andrzej Zikowski Antoni Ralski Nikolay A Paramonov Evgeny V Vinogradov Yuriy A Knirel Wiesaw Kaca |
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Institution: | Maciej Cedzyñski,Anna St Świerzko,Andrzej Zi´łkowski,Antoni Różalski,Nikolay A. Paramonov,Evgeny V. Vinogradov,Yuriy A. Knirel,Wiesław Kaca |
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Abstract: | A marked serological cross-reactivity was observed by ELISA and a precipitation test between anti-Proteus mirabilis O23 serum and the lipopolysaccharide as well as the O-specific polysaccharide from the Proteus mirabilis strain belonging to serogroup O6. The structures of the O-specific polysaccharides were elucidated using chemical and NMR spectroscopic analyses, and the only common component, 2-acetamido-2-deoxy-β-d -glucopyranose (β-d -GlcNAc), was revealed, which was suggested to be responsible for the cross-reactivity observed. Both anti-O23 and anti-O6 sera were shown to react with 1, 3-linked β-d -GlcNAc-containing O-antigen from Salmonella enterica ssp. arizonae O59 also. The lack of reactivity of Smith-degraded P. mirabilis O6 O-specific polysaccharide with homologous antiserum indicated the crucial role of α-d -glucuronic acid in specific antibody binding. |
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Keywords: | proteus lipopolysaccharide o-specific polysaccharide N-acetylglucosamine |
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