Confirmation of intraspecific crossing and single and joint segregation of biochemical loci ofVolvariella volvacea |
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| Affiliation: | 1. Department of Plant Pathology, Pennsylvania State University, University Park, Pennsylvania 16802 USA;2. Cornell Laboratory for Ecological and Evolutionary Genetics, Section of Ecology and Systematics, Cornell University, Ithaca, New York 14853 USA;1. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, PR China;2. College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, PR China;1. Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China;2. Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China;1. College of Food Science & Institute of Food Biotechnology, South China Agriculture University, Guangzhou 510640, China;2. Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510640, China;1. National Engineering Research Center of Edible Fungi, Ministry of Science and Technology, Shanghai 201403, China;2. Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, China;3. Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China;4. Institute of Edible Fungi, Shanghai Academy of Agriculture Science, Shanghai 201403, China;5. Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 470043, China |
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| Abstract: | Nineteen lines ofV. volvacea were obtained from worldwide sources and analyzed for allozyme activity. Twelve loci (Aat, Acp-2, Dia, Est-4, Gipi, Lap-1, Pep-GL, Pep-LLL-1, Pep-LLL-2, Pgm, Pgd, andSod) were monomorphic; 5 loci (Ada, Gpt, Mpi, Np, andPep-PAP) were polymorphic. Nine unique genotypes were distinguished among the 19 lines. Multilocus enzyme electrophoresis was used to confirm intraspecific crosses between putative homokaryons. Putative homokaryons were recognized by electrophoresis of single-spore-derived cultures and used as breeding stock. These breeding stocks were grown together in dual culture on agar, and selections were made from the interaction zone. These selections were then transferred to make spawn. Intraspecific crosses were confirmed by the presence of heteromeric allozymes. To determine single and joint segregation of biochemical loci, 84 single-spore-derived offspring from a confirmed hybrid sporocarp with five heterozygous loci were analyzed. Mendelian segregation was verified for the five loci. One linkage (Np withGpt,r=0.0) was found among 10 pairwise comparisons for joint segregation. The basidiospores ofV. volvacea are haploid and homokaryotic isolates may mate to yield heterokaryons. |
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