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Lipid and biomass production by the halotolerant microalga Nannochloropsis salina
Institution:1. Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV Wageningen, The Netherlands;2. FeyeCon Development & Implementation, Rijnkade 17a, 1382 GS Weesp, The Netherlands;1. Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, People''s Republic of China;2. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, People''s Republic of China;3. ShanghaiTech University, 100 Haike Road, Shanghai 201210, People''s Republic of China;4. School of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, People''s Republic of China;5. School of Pharmaceutical Science, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, People''s Republic of China
Abstract:The effect of environmental factors on cell-lipid content, on the growth rate and on the overall productivity of Nannochloropsis salina was tested in the laboratory and in outdoor cultures. Under optimum conditions in the laboratory, the maximum growth rate (μmax) was 0·030 h−1, which corresponds to a doubling time of 23 h. Cellular lipid content was affected by the phase of growth and the temperature, but not by nitrogen starvation, pH or the source of sea water. The most important factor affecting the output rate of biomass was the cell concentration. The maximum biomass productivity obtained in outdoor ponds was 24·5 g·m−2·day−1, and the lipid production rate was 4·0 g m−2·day−1.
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