Developing reverse genetics systems of northern cereal mosaic virus to reveal superinfection exclusion of two cytorhabdoviruses in barley plants |
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Authors: | Xiao‐ Dong Fang,Ji‐ Hui Qiao,Ying Zang,Qiang Gao,Wen‐ Ya Xu,Dong‐ Min Gao,Yi‐ Zhou Yang,Liang Xie,Ying Wang,Xian‐ Bing Wang |
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Affiliation: | 1. State Key Laboratory of Agro‐Biotechnology, College of Biological Sciences, China Agricultural University, Beijing China ; 2. College of Plant Protection, China Agricultural University, Beijing China |
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Abstract: | Recently, reverse genetics systems of plant negative‐stranded RNA (NSR) viruses have been developed to study virus–host interactions. Nonetheless, genetic rescue of plant NSR viruses in both insect vectors and monocot plants is very limited. Northern cereal mosaic virus (NCMV), a plant cytorhabdovirus, causes severe diseases in cereal plants through transmission by the small brown planthopper (SBPH, Laodelphax striatellus) in a propagative manner. In this study, we first developed a minireplicon system of NCMV in Nicotiana benthamiana plants, and then recovered a recombinant NCMV virus (rNCMV‐RFP), with a red fluorescent protein (RFP) insertion, in SBPHs and barley plants. We further used rNCMV‐RFP and green fluorescent protein (GFP)‐tagged barley yellow striate mosaic virus (rBYSMV‐GFP), a closely related cytorhabdovirus, to study superinfection exclusion, a widely observed phenomenon in dicot plants rarely studied in monocot plants. Interestingly, cellular superinfection exclusion of rBYSMV‐GFP and rNCMV‐RFP was observed in barley leaves. Our results demonstrate that two insect‐transmitted cytorhabdoviruses are enemies rather than friends at the cellular level during coinfections in plants. |
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Keywords: | cytorhabdovirus, monocot plants, NCMV, plant negative‐ stranded RNA viruses, reverse genetics systems, superinfection exclusion (SIE) |
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