几种放射孢子虫的形态特征和分子鉴定

为阐明黏孢子虫病的传播途径,在2018-2019年期间作者调查了异育银鲫养殖池塘,从底栖寡毛类苏氏尾鳃蚓中共检测出10种放射孢子虫.基于形态特征鉴定,10种放射孢子虫分别属于6个集合类群,其中雷氏放射孢子虫1种、桔瓣放射孢子虫2种、新放射孢子虫2种、三突放射孢子虫2种、棘放射孢子虫2种和匈牙利放射孢子虫1种;这些物种中...

MORPHOLOGICAL CHARACTERS AND DNA IDENTIFICATION OF SEVERAL ACTINOSPOREAN COLLECTED FROM OLIGOCHAETE BRANCHIURA SOWERBYI

Myxozoans are common microparasites in wild and farmed fish,and some species cause severe disease in cultured gibel carp.During the investigation of actinospores in oligochaetes from the gibel carp ponds form April 2018 to July 2019,nine actinospore types were found in the oligochaete Branchiura sowerbyi Beddard.Based on morphological characteristics,they were identified into six collection groups,including one Raabeia-type,two Aurantiactinomyxon-types,two Neoactinomyxum-types,two Triactinomyxon-types,two Echinactinomyxon-types and one Hungactinomyxon-type.The present study described six of them,Triactinomyxon type HZ,Triactinomyxon type DF,Echinactinomyxon type LY1,Echinactinomyxon type LY2,Neoactinomyxum type LY1 and Hungactinomyxon type DF.Blastn search using SSU rDNA gene sequences indicated that Triactinomyxon type HZ and Triactinomyxon type DF have the highest 93.39% and 94.48% homology similarity with Triactinomyxon type CZ,respectively.Echinactinomyxon type LY1 and Myxobolus lentisuturalis have the highest similarity(96.88%,AY119688);Echinactinomyxon type LY2 has the highest similarity with Hungactinomyxon type 1(97.86%,AY779062);Neoactinomyxum type LY has the highest similarity with Thelohanellus sp.(92.48%,MK412938);Hungactinomyxon type DF has the highest similarity with Hungactinomyxon(99.41%—99.64%).Phylogenetic analyses showed that Triactinomyxon type HZ and Triactinomyxon type DF clustered in the Chloromyxum subclade;Echinactinomyxon type LY1 and Echinactinomyxon type LY2 clustered in the Myxobolus clade;the Neoactinomyxum type LY1 clustered in the Thelohanellus clade.