Sequence composition similarities with the 7SL RNA are highly predictive of functional genomic features |
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Authors: | Yanick Paquet Alan Anderson |
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Institution: | 1.Centre de recherche en cancérologie de l’Université Laval, L’Hôtel-Dieu de Québec, Centre hospitalier universitaire de Québec, Québec G1R 2J6 and 2.Département de biologie, Université Laval, Québec G1K 7P4, Canada |
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Abstract: | Transposable elements derived from the 7SL RNA gene, such as Alu elements in primates, have had remarkable success in several mammalian lineages. The results presented here show a broad spectrum of functions for genomic segments that display sequence composition similarities with the 7SL RNA gene. Using thoroughly documented loci, we report that DNaseI-hypersensitive sites can be singled out in large genomic sequences by an assessment of sequence composition similarities with the 7SL RNA gene. We apply a root word frequency approach to illustrate a distinctive relationship between the sequence of the 7SL RNA gene and several classes of functional genomic features that are not presumed to be of transposable origin. Transposable elements that show noticeable similarities with the 7SL sequence include Alu sequences, as expected, but also long terminal repeats and the 5′-untranslated regions of long interspersed repetitive elements. In sequences masked for repeated elements, we find, when using the 7SL RNA gene as query sequence, distinctive similarities with promoters, exons and distal gene regulatory regions. The latter being the most notoriously difficult to detect, this approach may be useful for finding genomic segments that have regulatory functions and that may have escaped detection by existing methods. |
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