A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection |
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Authors: | Yuntao Zhou Qing Huang Jimin Gao Jianxin Lu Xizhong Shen Chunhai Fan |
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Affiliation: | 1.Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, 2.Wenzhou Medical College, Zhejiang 325035 and 3.Zhongshan Hospital, Fudan University, Shanghai 200032, China |
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Abstract: | We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples. |
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