Pythium intermedium,a species complex consisting of three phylogenetic species found in cool-temperate forest ecosystems |
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| Affiliation: | 1. College of Life Sciences, Shaanxi Normal University, Xi''an, 710119, China;2. River Basin Research Center, Gifu University, Gifu, 501-1193, Japan;3. Faculty of Regional Environment Science, Tokyo University of Agriculture, Tokyo, 156-8502, Japan;4. Life Science Research Center, Gifu University, Gifu, 501-1193, Japan;1. Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;2. Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;1. Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, China;2. Key Laboratory for Biology of Vegetable Diseases and Insect Pests of Shandong Province, Department of Plant Pathology, Shandong Agricultural University, Tai’an, 271018, Shandong, China;1. Department of Civil, Environmental and Mechanical Engineering, University of Trento, Via Mesiano, 20, 38123, Trento, TN, Italy;2. Center Agriculture Food Environment, University of Trento, via E. Mach 1, 38010, San Michele all’Adige, TN, Italy;3. Research and Innovation Centre, Fondazione Edmund Mach (FEM), via E. Mach 1, 38010, San Michele all’Adige, TN, Italy;4. Bi-PA nv (Biological Products for agriculture), Technologielaan 7, B-1840, Londerzeel, Belgium;1. Department of Geography, University of Cambridge, Cambridge, CB2 3EN, UK;2. Global Change Research Institute of the Czech Academy of Sciences (CzechGlobe), 603 00, Brno, Czech Republic;3. Department of Geography, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic;4. Swiss Federal Research Institute (WSL), 8903, Birmensdorf, Switzerland;5. Chair of Forest Growth and Dendroecology, University of Freiburg, 79106, Freiburg i.Br., Germany;6. Deutsche Trüffelbäume, 78315, Radolfzell, Germany;7. Archaeological Heritage Office Saxony, 01109, Dresden, Germany;1. Department of Biotechnology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan;2. Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan |
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| Abstract: | Pythium intermedium plays a vital role in the carbon cycle of cool-temperate forests and is widely distributed in Japan's forest soils. In this study, we performed a phylogenetic analysis of the P. intermedium species complex using DNA sequences from multiple loci. The study included 35 isolates from cool-temperate forest soils, seven known P. intermedium isolates, and six known Pythium attrantheridium isolates. We also performed morphological observations and mating tests. Our results showed that all the isolates formed one large clade but were divided into three subclades. Furthermore, we observed many mating reactions between isolates from different subclades, including between P. attrantheridium and P. intermedium. Therefore, we suggest that P. intermedium, P. attrantheridium, and another phylogenetic species belong to one species complex. This is the first report of a species complex within P. intermedium and will be helpful in understanding the evolution of Pythium species in natural ecosystems. |
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| Keywords: | Phylogenetic analysis Mating tests |
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