The Role of Podoplanin in the Biology of Differentiated Thyroid Cancers |
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| Authors: | Magdalena Rudzińska Damian Gawe? Justyna Sikorska Kamila M Karpińska Miros?aw Kiedrowski Tomasz St?pień Magdalena Marchlewska Barbara Czarnocka |
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| Institution: | 1. Department of Biochemistry and Molecular Biology, Center of Postgraduate Medical Education, Warsaw, Poland.; 2. Department of Pathology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.; 3. Department of General and Endocrinological Surgery, Copernicus Memorial Hospital, Łódź, Poland.; Institut de Génomique Fonctionnelle de Lyon, France, |
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| Abstract: | Podoplanin (PDPN), a mucin-type transmembrane glycoprotein specific to the lymphatic system is expressed in a variety of human cancers, and is regarded as a factor promoting tumor progression. The purpose of this study was to elucidate the molecular role of PDPN in the biology of thyroid cancer cells. PDPN expression was evaluated in primary thyroid carcinomas and thyroid carcinoma cell lines by RT-qPCR, Western blotting, IF and IHC. To examine the role of podoplanin in determining a cell''s malignant potential (cellular migration, invasion, proliferation, adhesion, motility, apoptosis), a thyroid cancer cell line with silenced PDPN expression was used. We observed that PDPN was solely expressed in the cancer cells of 40% of papillary thyroid carcinoma (PTC) tissues. Moreover, PDPN mRNA and protein were highly expressed in PTC-derived TPC1 and BcPAP cell lines but were not detected in follicular thyroid cancer derived cell lines. PDPN knock-down significantly decreased cellular invasion, and modestly reduced cell migration, while proliferation and adhesion were not affected. Our results demonstrate that PDPN mediates the invasive properties of cells derived from papillary thyroid carcinomas, suggesting that podoplanin might promote PTC progression. |
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