Potential innate immunity-related markers of endometrial receptivity and recurrent implantation failure (RIF) |
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| Institution: | 1. Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, The Medical School, University of Sheffield, Sheffield, UK;2. Biomolecular Science Research Centre, Department of Bioscience and Chemistry, Sheffield Hallam University, Sheffield, UK;3. Corporate Information and Computing Services, University of Sheffield, Sheffield, UK;4. Institute of Clinical Medicine, Department of Obstetrics and Gynaecology, University of Tartu, Tartu, Estonia;5. Competence Centre on Health Technologies, Tartu, Estonia;6. Department of Comparative Biomedical Sciences, Royal Veterinary College, University of London, London, UK;7. Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006, Tartu, Estonia;8. Institute of Genomics, University of Tartu, Tartu, Estonia;9. Institute of Biomedicine and Translational Medicine, Department of Pathophysiology, University of Tartu, Tartu, Estonia;1. Department of Obstetrics, Jingmen First People''s Hospital, Jingmen City, 448000, Hubei Province, China;2. Department of Gynecology, Jingmen First People''s Hospital, Jingmen City, 448000, Hubei Province, China;1. Key Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Wuhan, 430064, Hubei, China;2. Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Wuhan, 430064, Hubei, China;3. College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China;1. CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France;2. Union Evolution, Noyal-Sur-Vilaine, France;3. INRAE, Université de Tours, CHU de Tours, PIXANIM, 37380 Nouzilly, France;4. INRAE, Université de Tours, ISP, 37380 Nouzilly, France;5. Tours University, Tours, France;1. Department of Thyroid and Breast Surgery, the Affiliated Peoples Hospital of Ningbo University, Ningbo City, Zhejiang Province, 315040, China;2. Department of Nail Breast Surgery, Huai''an Second People''s Hospital, Huai''an City, Jiangsu Province, 223002, China;1. Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China;2. Department of Biochemistry and Molecular Biology, GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China;1. Department of Obstetrics and Gynaecology, Faculty of Medicine, Albert Szent-Györgyi Medical and Pharmaceutical Centre, University of Szeged, Szeged, Hungary;2. Department of Laboratory Medicine, Faculty of Medicine, Albert Szent-Györgyi Medical and Pharmaceutical Centre, University of Szeged, Szeged, Hungary;3. Department of Obstetrics and Gynaecology, Danderyd Hospital, Stockholm, Sweden |
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| Abstract: | The successful implantation of the embryo into a receptive endometrium is essential for the establishment of a viable pregnancy while recurrent implantation failure (RIF) is a real challenge in assisted reproduction. The maternal innate immune system, specifically the Toll-like receptors (TLRs), are involved in maintaining immunity in the female reproductive tract (FRT) required for fertility. In this study, we aimed to investigate the importance of innate immunity-related gene expression in the regulation of human fertility and as a prediction of potential outcome of in vitro fertilization - embryo transfer (IVF-ET), thus, we assessed the gene expression levels of TLR signalling molecules using quantitative real-time PCR between endometrial biopsies of healthy fertile women, and the patients experiencing RIF. Interestingly, our results showed that, TRIB2 and TLR9 genes were differentially expressed between the endometrial biopsies of healthy women and those with RIF. However, comparing expression levels of same genes between pre-receptive and receptive healthy endometrial biopsies showed different genes (ICAM1, NFKBIA, VCAM1, LIF, VEGFB, TLR5) had significantly altered expression, suggesting their involvement in endometrial receptivity. Thus, further investigations will enable us to better understand the role of these genes in the biology of FRT and as a possible target for the improvement of infertility treatments and/or development of non-hormonal contraception. |
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| Keywords: | Innate immune system Embryo implantation Recurrent implantation failure Toll-like receptors Endometrial receptivity |
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